Nature’s SAFE is Europe’s first charitable living biobank dedicated to storing tissues from animals threatened with extinction to halt and reverse biodiversity loss, protecting and restoring vital animal species. We collect, indefinitely store and regenerate reproductive cells and cell lines from multiple taxa, from tissue that would otherwise be disposed of. Cells stored with Nature’s SAFE remain in a dormant state until thawed when they can be defrosted for direct use in artificial reproductive technologies, or for cell culture. Uniquely, our science-led initiative unites world leading technology with species management to stop vital genetic diversity from being lost. We envisage a future with multiple satellite living biobanks around the world.